Construction and Characterization of 1F5 Chimeric Anti-CD20 Monoclonal Antibodies: an Efficient Splicing by Overlap Extension-polymerase Chain Reaction Technique
نویسندگان
چکیده
منابع مشابه
Dual Promoter Vector Construction for Simultaneous Gene Expression Using Spliced Overlap Extension by Polymerase Chain Reaction (SOE-PCR) Technique
There are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. Bicistronic system has disadvantages e.g. compared with two-promoter system. In this paper, a simple method based on spliced overlap extension by polymerase chain reaction (SOE-PCR) technique was demonstrat...
متن کاملResistance of Cloned 1F5 Chimeric Anti-CD20 Antibody Heavy-Chain Gene to DNA Polymerase due to a Predicted Hairpin Structure
Background: Formation of secondary structure such as DNA hairpins or loops may influence molecular genetics methods and PCR based approaches necessary for genetic engineering, in addition to gene regulation. Materials and Methods: A polymerase chain reaction with splice overlap extension (SOE-PCR) was used to create fully synthetic 1F5 chimeric anti-CD20 heavy- and light-chain genes. The chi...
متن کاملdual promoter vector construction for simultaneous gene expression using spliced overlap extension by polymerase chain reaction (soe-pcr) technique
there are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. bicistronic system has disadvantages e.g. compared with two-promoter system. in this paper, a simple method based on spliced overlap extension by polymerase chain reaction (soe-pcr) technique was demonstrat...
متن کاملresistance of cloned 1f5 chimeric anti-cd20 antibody heavy-chain gene to dna polymerase due to a predicted hairpin structure
background: formation of secondary structure such as dna hairpins or loops may influence molecular genetics methods and pcr based approaches necessary for genetic engineering, in addition to gene regulation. materials and methods: a polymerase chain reaction with splice overlap extension (soe-pcr) was used to create fully synthetic 1f5 chimeric anti-cd20 heavy- and light-chain genes. the chimer...
متن کاملproduction and characterization of new anti-human cd20 monoclonal antibody
the b-cell cd20 antigen is one of the most reliable surface targets in immunotherapy of b lymphoma. in this project, we studied the production and characterization of a new monoclonal antibody against chimeric human cd20 extra loops (hcd20 exl). the results showed that clone c12h, igg2/k isotype reacted with the antigen in elisa and immunoblot. the kd value was found to be 2×10-9m and flow cyt...
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ژورنال
عنوان ژورنال: Iranian Journal of Allergy, Asthma and Immunology
سال: 2021
ISSN: 1735-5249,1735-1502
DOI: 10.18502/ijaai.v20i2.6054